Recombinant Rat Clu, His-tagged

• Cat.No.: Clu-3664R
• Product Overview: Total 240 AA. MW: 26.5 kDa (calculated). N-Terminal T7-Tag and C-Terminal His-tag, 25 extra AA (highlighted).

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: Clusterin is a 75-80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the Clu gene.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.01M Tris pH 7.2.
• Research topic: Animal studies; Others; Renal disease; Sepsis
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA, Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS-PAGE to determine purity of the protein
• Sequences of amino acids: MASMTGGQQM GRDPNSSSPF YFWMNGDRID SLLESDRQQS QVLDAMQDSF TRASGIIDTL FQDRFFTHEP QDIHHFSPMG FPHKRPHLLY PKSRLVRSLM PLSHYGPLSF HNMFQPFFDM IHQAQQAMDV QLHSPALQFP DVDFLKEGED DRTVCKEIRH NSTGCLKMKG QCEKCQEILS VDCSTNNPAQ ANLRQELNDS LQVAERLTQQ YNELLHSLQS KMLNTSSLLE QALEHHHHHH
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Clu clusterin [ Rattus norvegicus ]
• Official Symbol: Clu
• Synonyms: Clu; clusterin; CLI; DAG; APOJ; SGP2; SP40; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B; sulfated glycoprotein 2; dimeric acid glycoprotein; testosterone-repressed prostate message; testostrone-repressed prostate message 2; testosterone repressed prostate message 2
• Gene ID: 24854
• mRNA Refseq: NM_053021
• Protein Refseq: NP_444180
• UniProt ID: P05371
• Chromosome Location: 15p12
• Pathway: Formation of Platelet plug; Hemostasis; Platelet Activation; Platelet degranulation; Response to elevated platelet cytosolic Ca2+
• Function: NOT ATPase activity; ATPase activity; misfolded protein binding; ubiquitin protein ligase binding; protein binding