Recombinant Human PTK-2 Protein Tyrosine Kinase 2, His-tagged, Active

• Cat.No.: PTK2-309H
• Product Overview: Recombinant human FAK (393-698) was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=35kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 393-698 a.a.
• Description: FAK (Focal Adhesion Kinase ) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness. Elevated FAK expression in anaplastic astrocytoma and glioblastoma tumor biopsy samples has been demonstrated.
• Sequence: 393-698.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PTK2 PTK2 protein tyrosine kinase 2 [ Homo sapiens ]
• Synonyms: PTK2; PTK-2 protein tyrosine kinase 2; FAK; FADK; FAK1; pp125FAK; Focal adhesion kinase 1; FADK 1; Protein-tyrosine kinase 2; Protein-tyrosine kinase 2; EC 2.7.10.2
• Gene ID: 5747
• mRNA Refseq: NM_005607
• Protein Refseq: NP_005598
• MIM: 600758
• UniProt ID: Q05397
• Chromosome Location: 8q24-qter
• Pathway: Axon guidance; ErbB signaling pathway; Chemokine signaling pathway; Focal adhesion; Leukocyte transendothelial migration; Pathways in cancer; Regulation of actin cytoskeleton; Small cell lung cancer; VEGF signaling pathway; Apoptosis; Axon guidance; Hemostasis; Integrin cell surface interactions
• Function: ATP binding; SH2 domain binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; signal transducer activity; transferase activity

Citation

Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling

Journal: Cell Research    PubMed ID: 30514903    Data: 2019/1/1

Authors: Xingguang Liu, Peng Zhang, Xuetao Cao

Article Snippet:After washing 3 times, the precipitates were subjected to immunoblot analysis.After washing 3 times, the precipitates were subjected to immunoblot analysis.. In vitro kinase assay Purified active IKKβ or TBK1 protein (Abcam) was preincubated with active Pyk2 protein (Creative BioMart) in ice water for 15 min. Then MBP and a magnesium-ATP ‘cocktail’ (Upstate) were added, followed by incubation for 20 min at 30 °C.. Samples were subjected to immunoblot analysis with antibody to phosphorylated IKKβ, TBK1 or MBP.Samples were subjected to immunoblot analysis with antibody to phosphorylated IKKβ, TBK1 or MBP.