||Recombinant bovine IGFBP-2 produced inCHO cellsis a 284 amino acid protein. MW = 30.776 kDa.
||Bovine Insulin-like Growth Factor Binding Protein-2 (bIGFBP-2) is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. bIGFBP-2 binds both human and bovine IGF-I and IGF-II and modifies their biological activity and bioavailability. The Animal/Media Grade product contains mature bovine IGFBP-2 of correct Molecular Weight as the major species. As a result of alternative processing by the CHO cells used for the bIGFBP-2 production, three other minor bIGFBP-2 isoforms are also present: 1) One with a three amino acid C-terminal deletion, denoted Des(1-3) bIGFBP-2. 2) One with a single amino acid N-terminal extension, denoted A-bIGFBP-2. 3) One with a four amino acid N-terminal extension, denoted GARA-bIGFBP-2.
||>95% by HPLC and Mass Spectrometry.
||IGF-I Binding Assay : ED50 < 10 nM.
|State And Appearance:
||Lyophilized white powder dried from 0.1% TFA / 30% acetonitrile and stored under dry nitrogen at a slight vacuum (-25 kPa).
||At least 2 years at 2 - 4°C (lyophilized).