| Cat. No. : |
BTC-320B |
| Description : |
Bovine Betacellulin (BTC), a member of the EGF family, is an 80 amino acid heparin-binding protein. Like other members of the EGF family, it is synthesized as a transmembrane precursor. The soluble cytokine, containing one EGF structural motif, is released by proteolytic cleavage. BTC can bind to the EGF receptor (ErbB-1) which then dimerizes with another member of the ErbB receptor family to signal through the tyrosine kinase pathway. BTC is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells and is the only member of the EGF family to stimulate insulin secretion in pancreatic b cells. |
| Source : |
E. coli. |
| Purity : |
By N-terminal sequencing: >95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC. |
| Molecular Weight : |
Theoretical: 8995 daltons. By Mass Spectrometry the product is 50% [Gly1]BTC (9052 daltons) and 50% Des(1-4)BTC (8709 daltons). |
| N-terminal Sequence Analysis : |
5 residues >95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC. |
| Activity : |
Cell growth bioassay in Balb/c 3T3 fibroblasts: ED50<10 ng/ml. |
| Endotoxin : |
<0.1 EU/µg. |
| State And Appearance : |
Lyophilized white powder. Dried from 0.05 M acetic acid, 0.05% (v/v) trifluoroacetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa). |
| Storage : |
At least 2 years at 2 – 4°C(lyophilized). |
