|Cat. No. :
|Product Overview :
||Recombinant human full-length CDK9 and CyclinK were co-expressed by baculovirus inSf9insect cells using an N-terminal GST tag on both proteins. MW=68 kDa and CyclinK~67 kDa.
||CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle. CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
||Sample Purity Data. For specific information on a given lot, see related technical data sheet.
|Storage And Stability :
||Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.