||Recombinant full-length human PRKCZ was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=93kDa.
||PKCζ is an atypical isoform of the PKC family. PKCζ is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Overexpression of PKCζ and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation. Overexpression of PKCζ also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCζ is an upstream modulator of p70S6K, an important regulator of cell proliferation.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.