Cat. No.: |
TEK-445H |
Product Overview: |
Recombinant human TIE 2 (771-end) was expressed by baculovirus inSf9insect cells using an N-terminal GST tag. MW = 61 kDa. |
Description: |
TIE 2 or TEK is a receptor tyrosine kinase that is expressed principally on vascular endothelium. Disrupting TIE 2 function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TIE 2 receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TIE 2 kinase domain and are part of the cellular responses that mediate TIE 2 function. TIE 2 expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TIE 2 is also overexpressed in areas of active angiogenesis in normal tissues. |
Source: |
Sf9 insect cells using baculovirus. |
Sequence: |
771-end. |
Applications: |
Kinase Assay, Western Blot. |
Storage And Stability: |
Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |