||Recombinant full-length human TSSK2 was expressed by baculovirus inSf9insect cells using an N-terminal GST tag. MW = 66kDa.
||TSSK2 is an intronless serine-threonine kinase that was originally detected on mouse chromosome 16. TSSK2 and TSSK1 share 72% overall amino acid identity and 83% identity in the kinase domain. Real-time PCR showed abundant TSSK2 expression in testis and lower levels in heart, brain, and placenta while Western blot analysis detected TSSK2 at the predicted molecular mass of 40kDa in human sperm and testis. Co-immunoprecipitation and yeast 2-hybrid analysis show that TSSK2 can interact with a protein TSKS, that also acts as a substrate for this protein kinase.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.