"ATP6V1E1" Related Products

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Recombinant Human ATP6V1E1, T7-tagged

Cat. No.: ATP6V1E1-386H
Product Overview: Recombinant human ATP6V1E1 (aa. 1-226) produced inE.coliis a 26 kDa protein. It was fused to T7 tag at N-terminus.
Description: This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of a hexamer of three A and three B subunits plus the C, D, and E subunits. It contains the ATP catalytic site. The protein is known as the E subunit and is found ubiquitously.
Source: E.coli.
Purity: 95%.
Preparation: 0.1mg/0.073 ml.
Tested Application: E, WB, MS.
Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.
Species Reactivity: n/aH.
Storage: Store at -70°C with any protein, exposing ATP6V1E1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Pathways: Epithelial cell signaling in Helicobacter pylori infection; Metabolic pathways; Oxidative phosphorylation; Vibrio cholerae infection
Gene Name: ATP6V1E1 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 [ Homo sapiens ]
Synonyms: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; P31; Vma4; ATP6E; ATP6E2; ATP6V1E; ATP6V1E1; vacuolar H+ ATPase E1; V-ATPase, subunit E; H+-transporting ATP synthase chain E, vacuolar; H(+)-transporting two-sector ATPase, 31kDa subunit; ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31Kd; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1; V-ATPase 31 kDa subunit; V-ATPase subunit E 1; Vacuolar proton pump subunit E 1
Gene ID: 529
mRNA Refseq: NM_001039366
Protein Refseq: NP_001034455
MIM: 108746
UniProt ID: P36543
Chromosome Location: 22q11.1
Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrolase activity; protein binding; proton-transporting ATPase activity, rotational mechanism

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