Recombinant Mouse NEL-like 2 (Chicken), His-tagged

• Cat.No.: Nell2-394M
• Product Overview: Recombinant Mouse Nell2 was expressed inE.Coliusing an N-terminal His tag. MW=28 kDa.

Specification

• Cat. No.: Nell2-394M
• Description: NELL2 is a neuron-specific thrombospondin-1-like extracellular protein containing six epidermal growth factor-like domains. It is highly expressed in the hippocampus in vivo and promotes survival of rat primary cultured neurons through activation of JNK and suppression of ERK. As ERK is required for the induction of hippocampal long term potentiation (LTP) and JNK appears to be inhibitory on LTP, it had been suggested that NELL2 may play a role in synaptic plasticity.
• Source: E.Coli.
• Sequence: 2-238.
• Purity: ~95%.
• Specific Activity: n/a.
• Applications: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
• Buffer: 100mM sodium phosphate, 10mM Tris, 500mM NaCl, 25 mM imidazole, 2mM MgCl2, 10% glycerol.
• Storage: Store at -70˚C. As with any protein, exposing Nell2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS-PAGE analysis of recombinant NELL2 fragment on Coomassie Blue-stained 12% SDS-PAGE gel.

Gene Information

• Gene Name: Nell2 NEL-like 2 (chicken) [ Mus musculus ]
• Synonyms: Nell2; NEL-like 2 (chicken); mel91; R75516; A330108N19Rik; nel-like 2 homolog
• Gene ID: 54003
• mRNA Refseq: NM_016743
• Protein Refseq: NP_058023
• UniProt ID: Q61220
• Chromosome Location: 15
• Function: calcium ion binding; structural molecule activity