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Recombinant Murine Interleukin 12a, P35-P40

Cat.No. : Il12a-211M
Product Overview : Recombinant Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDA (p35) and 40 kDa (p40) subunits. It is expressed inCHO cells.
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Cat. No. : Il12a-211M
Description : Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+ T cells and NK cells. Human IL-12 also promotes the development of proinflammatory/TH1-like CD4+ cells and cytotoxic CD8+T cells.
Source : CHO cells.
Purity : Greater than 98% (determined by SDS-PAGE and HPLC analysis).
Specific Activity : ≥ 1 x10⁷ IU/mg.
Endotoxin Level : < 0.1 ng per µg of rMu IL-12 (1EU/µg).
Biological Activity : The ED₅₀ as determined by the dose-dependent stimulation of INF-( production by murine splenocytes co-stimulated with IL-18 is ≤ 0.1 ng / ml, corresponding to a specific activity of ≥ 1 x10⁷ units/mg.
Formulation : Lyophilized from a sterile-filtered protein solution in 0.5 x PBS (pH 7.2).
Reconstitution : Please Note: Always centrifuge product before opening vial. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can than be diluted into other aqueous buffers and stored at -20°C for future use.
Storage : The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted murine IL-12 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Gene Name : Il12a interleukin 12a [ Mus musculus ]
Synonyms : Il12a; interleukin 12a; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232; interleukin 12a p35 subunit; interleukin 12a
Gene ID : 16159
mRNA Refseq : NM_001159424
Protein Refseq : NP_001152896
UniProt ID : P43431
Chromosome Location : 3 E1; 3 37.0 cM
Pathway : Allograft rejection; Cytokine-cytokine receptor interaction; RIG-I-like receptor signaling pathway; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
Function : cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do mass spectrometry-based techniques reveal post-translational modifications on IL12A? 08/18/2022

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models? 06/12/2022

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners? 06/26/2021

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture? 12/17/2020

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors? 10/25/2020

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells? 05/19/2019

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors? 06/05/2018

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function? 03/08/2018

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains? 11/23/2017

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms? 02/16/2017

Bioluminescence imaging tracks IL12A expression dynamics in vivo.

Customer Reviews (3)

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Reviews
01/14/2021

    Suitable for co-immunoprecipitation.

    12/21/2020

      Consistent in bioactivity.

      11/16/2020

        Good for receptor binding studies.

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