||Recombinant human BCR (1-695) was expressed by baculovirus inSf9insect cellsusing an N-terminal GST tag. MW = 120kDa.
||BCR (also known as breakpoint cluster region) encodes a phosphoprotein that has serine/threonine kinase activity. The human c-ABL oncogene is translocated from chromosome 9 to a region on chromosome 22 (associated with BCR) in chronic myelocytic leukemia. In classical t(9;22) translocation, as observed in chronic granulocytic leukemia (CGL), a hybrid DNA unit is produced comprising the BCR gene product plus the translocated c-ABL gene from chromosome 9. The BCR-ABL hybrid protein (p210) is formed that displays increased tyrosine kinase activity. A similar DNA rearrangement of the p210 protein is also found in cases of Philadelphia-positive acute lymphoblastic leukemia (ALL).
||Sf9 insect cells using baculovirus.
|Storage And Stability:
||Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.