| Description : |
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
137.5 kDa |
| AA Sequence : |
MVDPVGFAEAWKAQFPDSEPPRMEL RSVGDIEQELERCKASIRRLEQEVN QERFRMIYLQTLLAKEKKSYDRQRW GFRRAAQAPDGASEPRASASRPQPA PADGADPPPAEEPEARPDGEGSPGK ARPGTARRPGAAASGERDDRGPPAS VAALRSNFERIRKGHGQPGADAEKP FYVNVEFHHERGLVKVNDKEVSDRI SSLGSQAMQMERKKSQHGAGSSVGD ASRPPYRGRSSESSCGVDGDYEDAE LNPRFLKDNLIDANGGSRPPWPPLE YQPYQSIYVGGMMEGEGKGPLLRSQ STSEQEKRLTWPRRSYSPRSFEDCG GGYTPDCSSNENLTSSEEDFSSGQS SRVSPSPTTYRMFRDKSRSPSQNSQ QSFDSSSPPTPQCHKRHRHCPVVVS EATIVGVRKTGQIWPNDGEGAFHGD ADGSFGTPPGYGCAADRAEEQRRHQ DGLPYIDDSPSSSPHLSSKGRGSRD ALVSGALESTKASELDLEKGLEMRK WVLSGILASEETYLSHLEALLLPMK PLKAAATTSQPVLTSQQIETIFFKV PELYEIHKEFYDGLFPRVQQWSHQQ RVGDLFQKLASQLGVYRAFVDNYGV AMEMAEKCCQANAQFAEISENLRAR SNKDAKDPTTKNSLETLLYKPVDRV TRSTLVLHDLLKHTPASHPDHPLLQ DALRISQNFLSSINEEITPRRQSMT VKKGEHRQLLKDSFMVELVEGARKL RHVFLFTDLLLCTKLKKQSGGKTQQ YDCKWYIPLTDLSFQMVDELEAVPN IPLVPDEELDALKIKISQIKNDIQR EKRANKGSKATERLKKKLSEQESLL LLMSPSMAFRVHSRNGKSYTFLISS DYERAEWRENIREQQKKCFRSFSLT SVELQMLTNSCVKLQTVHSIPLTIN KEDDESPGLYGFLNVIVHSATGFKQ SSNLYCTLEVDSFGYFVNKAKTRVY RDTAEPNWNELDPQALQDRDWQRTV IAMNGIEVKLSVKFNSREFSLKRMP SRKQTGVFGVKIAVVTKRERSKVPY IVRQCVEEIERRGMEEVGIYRVSGV ATDIQALKAAFDVNNKDVSVMMSEM DVNAIAGTLKLYFRELPEPLFTDEF YPNFAEGIALSDPVAKESCMLNLLL SLPEANLLTFLFLLDHLKRVAEKEA VNKMSLHNLATVFGPTLLRPSEKES KLPANPSQPITMTDSWSLEVMSQVQ VLLYFLQLEAIPAPDSKRQSILFST EVTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
