Active Recombinant Human CASP8 Protein

• Cat.No.: CASP8-26606TH
• Product Overview: Recombinant human CASP8 protein without tag was expressed in E. coli.

Specification

• Description: Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested for its ability to enzymatically cleave these two substrates Ac-IETD-pNA or Ac-IETD-AFC.
• Source: E. coli
• Species: Human
• Form: Lyophilized powder
• Bio-activity: Protein activity determined to be > 5000 units/mg. One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37 centigrade in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Purity: Purity was assessed by SDS-PAGE (≥95%) and by HPLC.
• Applications: Apuri, Blocking, ELISA, Functional Assay
• Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
• Storage: Store at -80 centigrade.
• Storage Buffer: Reconstitute to 1 unit per µl in PBS containing 15% glycerol.

Gene Information

• Gene Name: CASP8 caspase 8 [ Homo sapiens (human) ]
• Official Symbol: CASP8
• Synonyms: CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; CASP8; caspase 8; caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog; apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; EC 3.4.22.61
• Gene ID: 841
• mRNA Refseq: NM_001228
• Protein Refseq: NP_001219
• MIM: 601763
• UniProt ID: Q14790