Recombinant Mouse Caspase 8

• Cat.No.: Casp8-1645M
• Product Overview: Recombinant active mouse caspase-8 was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Casp8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, casp8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of casp8 consists of large and small subunits which associate to form the active enzyme.
• Biological Activity: 5000 units/mg
• Form: Lyophilized powder.
• Appearance: Lyophilized powder
• Reconstitution: Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
• Purity: ≥ 95 % by SDS-PAGE
• Storage: The lyophilized caspase-8 is stable for 1 year at -70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at -70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
• Pathways: Activation of Pro-Caspase 8; Activation; Alzheimer"s disease; Androgen Receptor Signaling Pathway; Apoptosis; Apoptosis Modulation by HSP70; Apoptotic cleavage of cellular proteins; Apoptotic execution phase; Death Receptor Signalling; Extrinsic Pathway for Apoptosis; Herpes simplex infection; Huntington"s disease; Intrinsic Pathway for Apoptosis; Legionellosis; MAPK signaling pathway

Gene Information

• Gene Name: Casp8 caspase 8 [ Mus musculus ]
• Official Symbol: Casp8
• Synonyms: Casp8; caspase 8; MACH; Mch5; FLICE; CASP-8; caspase-8; Fas-linked ICE-like protease; Caspase-8; EC 3.4.22.61
• Gene ID: 12370
• mRNA Refseq: NM_001080126
• Protein Refseq: NP_001073595
• UniProt ID: O89110
• Chromosome Location: 1 B; 1 30.1 cM
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; identical protein binding; peptidase activity; protein binding; tumor necrosis factor receptor binding