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Recombinant Human DCK Protein, His-tagged

Cat.No. : DCK-27365TH
Product Overview : Recombinant Human DCK protein (1-260aa), fused with His-tag, was expressed in E.coli.
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Description : DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Source : E. coli
Species : Human
Tag : His
Form : Liquid
Molecular Mass : 34.6 kDa
AA Sequence : MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSMATPPKRSCPSFSA SSEGTRIKKISIEGNIAAGKSTFVN ILKQLCEDWEVVPEPVARWCNVQST QDEFEELTMSQKNGGNVLQMMYEKP ERWSFTFQTYACLSRIRAQLASLNG KLKDAEKPVLFFERSVYSDRYIFAS NLYESECMNETEWTIYQDWHDWMNN QFGQSLELDGIIYLQATPETCLHRI YLRGRNEEQGIPLEYLEKLHYKHES WLLHRTLKTNFDYLQEVPILTLDVN EDFKDKYESLVEKVKEFLSTL
Purity : > 90% by SDS-PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/mL (determined by Bradford assay)
Storage Buffer : In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol
Gene Name : DCK deoxycytidine kinase [ Homo sapiens (human) ]
Official Symbol : DCK
Synonyms : DCK; deoxycytidine kinase; deoxycytidine kinase; deoxynucleoside kinase; EC 2.7.1.74
Gene ID : 1633
mRNA Refseq : NM_000788
Protein Refseq : NP_000779
MIM : 125450
UniProt ID : P27707

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How is DCK implicated in resistance to chemotherapy? 09/01/2022

Reduced DCK activity or expression can lead to resistance against nucleoside analog-based chemotherapy.

What therapeutic strategies are being explored to modulate DCK activity? 07/10/2021

Strategies include modulating DCK levels to overcome drug resistance and improve treatment outcomes.

What is the significance of DCK in the action of certain chemotherapeutic agents? 04/28/2021

DCK activation of prodrugs enhances their cytotoxicity, improving chemotherapeutic efficacy.

Are there known genetic variants of DCK associated with different responses to treatment? 09/14/2020

Genetic variants in DCK have been linked to varied responses to nucleoside analog drugs.

How does DCK contribute to nucleoside metabolism? 08/27/2018

It phosphorylates deoxyribonucleosides, contributing to their conversion to active triphosphate forms.

In which types of cells or tissues is DCK predominantly expressed? 06/17/2018

DCK is widely expressed but shows higher activity in proliferating cells and certain tissues like the liver.

What is the primary role of DCK in cellular processes? 02/22/2018

DCK plays a crucial role in activating nucleoside analog prodrugs.

Customer Reviews (3)

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Reviews
08/05/2020

    Worked as expected, pure.

    07/01/2020

      Excellent for research, quality.

      07/26/2018

        Fast shipping, reliable product.

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