Recombinant Human DCK Protein, His-tagged
Cat.No. : | DCK-27365TH |
Product Overview : | Recombinant Human DCK protein (1-260aa), fused with His-tag, was expressed in E.coli. |
- Specification
- Gene Information
- Related Products
Description : | DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid |
Molecular Mass : | 34.6 kDa |
AA Sequence : | MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSMATPPKRSCPSFSA SSEGTRIKKISIEGNIAAGKSTFVN ILKQLCEDWEVVPEPVARWCNVQST QDEFEELTMSQKNGGNVLQMMYEKP ERWSFTFQTYACLSRIRAQLASLNG KLKDAEKPVLFFERSVYSDRYIFAS NLYESECMNETEWTIYQDWHDWMNN QFGQSLELDGIIYLQATPETCLHRI YLRGRNEEQGIPLEYLEKLHYKHES WLLHRTLKTNFDYLQEVPILTLDVN EDFKDKYESLVEKVKEFLSTL |
Purity : | > 90% by SDS-PAGE |
Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/mL (determined by Bradford assay) |
Storage Buffer : | In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol |
Gene Name : | DCK deoxycytidine kinase [ Homo sapiens (human) ] |
Official Symbol : | DCK |
Synonyms : | DCK; deoxycytidine kinase; deoxycytidine kinase; deoxynucleoside kinase; EC 2.7.1.74 |
Gene ID : | 1633 |
mRNA Refseq : | NM_000788 |
Protein Refseq : | NP_000779 |
MIM : | 125450 |
UniProt ID : | P27707 |
Products Types
◆ Recombinant Protein | ||
DCK-2390H | Recombinant Human DCK Protein, GST-tagged | +Inquiry |
DCK-0824H | Recombinant Human DCK Protein (M1-L260), Tag Free | +Inquiry |
DCK-0825H | Recombinant Human DCK Protein (M1-L260), His/Strep tagged | +Inquiry |
DCK-1451R | Recombinant Rat DCK Protein, His (Fc)-Avi-tagged | +Inquiry |
DCK-2231M | Recombinant Mouse DCK Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
DCK-7051HCL | Recombinant Human DCK 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-0879 | dCK Screening Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionReduced DCK activity or expression can lead to resistance against nucleoside analog-based chemotherapy.
Strategies include modulating DCK levels to overcome drug resistance and improve treatment outcomes.
DCK activation of prodrugs enhances their cytotoxicity, improving chemotherapeutic efficacy.
Genetic variants in DCK have been linked to varied responses to nucleoside analog drugs.
It phosphorylates deoxyribonucleosides, contributing to their conversion to active triphosphate forms.
DCK is widely expressed but shows higher activity in proliferating cells and certain tissues like the liver.
DCK plays a crucial role in activating nucleoside analog prodrugs.
Customer Reviews (3)
Write a reviewWorked as expected, pure.
Excellent for research, quality.
Fast shipping, reliable product.
Ask a Question for All DCK Products
Required fields are marked with *
My Review for All DCK Products
Required fields are marked with *
Inquiry Basket