Recombinant Human DFFA, His-tagged

• Cat.No.: DFFA-27566TH
• Product Overview: ICAD recombinant full length protein (Human), with an N-terminal His-tag, ~48kDa.

Specification

• Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Conjugation: HIS
• Source: E. coli
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, PBS
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequence Similarities: Contains 1 CIDE-N domain.

Gene Information

• Gene Name: DFFA DNA fragmentation factor, 45kDa, alpha polypeptide [ Homo sapiens ]
• Official Symbol: DFFA
• Synonyms: DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, 45 kD, alpha polypeptide; DNA fragmentation factor subunit alpha; DFF 45; DFF1; DFF45; DNA fragmentation factor; 45 kD; alpha subunit; ICAD
• Gene ID: 1676
• mRNA Refseq: NM_004401
• Protein Refseq: NP_004392
• MIM: 601882
• Uniprot ID: O00273
• Chromosome Location: 1p36.3-p36.2
• Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem
• Function: deoxyribonuclease activity