Recombinant Murine Tumor Necrosis Factor

Cat. No.: Tnf-331M
Product Overview: Recombinant Murine Tumor Necrosis Factor-alpha (rMu TNF-alpha) is a pleiotrophic proinflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts expressed inSteptomyces lividans. MW=17.3kDa.
Description: TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-alpha is a soluble 156 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Source: Steptomyces lividans.
Quantity: 20 μg.
Specific Activity: ≥ 9 x 10⁸ IU/mg.
Purity: Greater than 98% (determined by SDS-PAGE and HPLC analysis).
Endotoxin Level: < 0.1 ng per μg of rMu TNF-alpha (1EU/μg).
Biological Activity: Recombinant human TNF-alpha is fully biologically active when compared to a standard. The ED₅₀, as determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is 0.05 ng/ml corresponding to a specific activity of ≥ 9 x 10⁸ units/mg.
Formulation: Sterile, lyophilized protein. Each mg contains 50 mM Tris-HCl (pH 8.0) and 20 mM NaCl.
Reconstitution: Please note: Always centrifuge product briefly before opening the vial. The lyophilized rMu TNFalpha should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be further diluted into other aqueous buffers and stored at -20°C for future use.
Storage And Stability: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu TNF-alpha protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Gene Name: Tnf tumor necrosis factor [ Mus musculus ]
Synonyms: Tnf; tumor necrosis factor; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; MGC151434; TNF-alpha; TNF alpha; tumor necrosis factor alpha; tumor necrosis factor-alpha
Gene ID: 21926
mRNA Refseq: NM_013693
Protein Refseq: NP_038721
UniProt ID: P06804
Chromosome Location: 19.06 cM
Pathway: Adipocytokine signaling pathway; Allograft rejection; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Natural killer cell mediated cytotoxicity; RIG-I-like receptor signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus; Type II diabetes mellitus
Function: cytokine activity; tumor necrosis factor receptor binding

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