||Recombinant human Heregulin (HRG) protein was expressed inE. coli. MW= 44kDa (full length) and 30kDa (extracellular domain).
||Heregulin (HRG) is a glycoprotein of 44kDa which binds to c-erbB-3 and c-erbB-4 receptors with low and high affinities, respectively. Heregulins constitute a family of mosaic proteins comprising of at least 12 members. They contain certain recognizable domains which include: an amino terminal (N-ter) region; an immunoglobulinlike motif (Ig); a glycosylated spacer sequence (glyco); an EGF-like motif (EGF), which is subdivided into α and β isoforms; a juxtamembrane region subdivided into isoforms 1-3; a hydrophobic transmembrane domain (TM); a common cytoplasmic tail (Cyto); and a variable-length cytoplasmic tail (a-c). Using this nomenclature, several isoforms of HRG are designated as HRG-α1, HRG-α2, HRG-α3, HRG-β1, HRG-β2, HRG-β3, HRG-α2b, etc.
||In SDS-PAGE, the purified recombinant Heregulin α1 protein appears as a single band at 30kDa. Heregulin α1 protein modulates the proliferation and differentiation of cultured mammary cells and stimulates the tyrosine kinase activity of ErbBs.
|Applications And Suggested Dilutions:
||Western Blotting, Stimulation of Tyrosine Kinase Activity of Erbs (Protein at 5nM); Cell Proliferation and Differentiation (Protein at 5nM: dilute 5μg of stock protein to 30ml of culture medium). The optimal dilution for a specific application should be determined by the investigator.
||T47-D breast cancer cells.
||Purified Heregulin α1 is packaged in physiological saline, pH 7.4, with 0.2% BSA. Note that this product is supplied without Sodium azide or any other preservative.
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 45 kDa.
|Storage And Stability:
||Store vial at -20°C to -70°C. When stored at the recommended temperature, this protein is stable for 24 months.