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Recombinant Human Histidine-Rich Glycoprotein

Cat. No.: HRG-332H
Product Overview: Recombinant human Heregulin (HRG) protein was expressed inE. coli. MW= 44kDa (full length) and 30kDa (extracellular domain).
Description: Heregulin (HRG) is a glycoprotein of 44kDa which binds to c-erbB-3 and c-erbB-4 receptors with low and high affinities, respectively. Heregulins constitute a family of mosaic proteins comprising of at least 12 members. They contain certain recognizable domains which include: an amino terminal (N-ter) region; an immunoglobulinlike motif (Ig); a glycosylated spacer sequence (glyco); an EGF-like motif (EGF), which is subdivided into α and β isoforms; a juxtamembrane region subdivided into isoforms 1-3; a hydrophobic transmembrane domain (TM); a common cytoplasmic tail (Cyto); and a variable-length cytoplasmic tail (a-c). Using this nomenclature, several isoforms of HRG are designated as HRG-α1, HRG-α2, HRG-α3, HRG-β1, HRG-β2, HRG-β3, HRG-α2b, etc.
Source: E. coli.
Comments: In SDS-PAGE, the purified recombinant Heregulin α1 protein appears as a single band at 30kDa. Heregulin α1 protein modulates the proliferation and differentiation of cultured mammary cells and stimulates the tyrosine kinase activity of ErbBs.
Applications And Suggested Dilutions: Western Blotting, Stimulation of Tyrosine Kinase Activity of Erbs (Protein at 5nM); Cell Proliferation and Differentiation (Protein at 5nM: dilute 5μg of stock protein to 30ml of culture medium). The optimal dilution for a specific application should be determined by the investigator.
Positive Control: T47-D breast cancer cells.
Supplied As: Purified Heregulin α1 is packaged in physiological saline, pH 7.4, with 0.2% BSA. Note that this product is supplied without Sodium azide or any other preservative.
Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 45 kDa.
Storage And Stability: Store vial at -20°C to -70°C. When stored at the recommended temperature, this protein is stable for 24 months.
Gene Name: HRG histidine-rich glycoprotein [ Homo sapiens ]
Synonyms: HRG; histidine-rich glycoprotein; HPRG; HRGP; DKFZp779H1622; thrombophilia due to elevated HRG; histidine-proline rich glycoprotein; Histidine-proline-rich glycoprotein
Gene ID: 3273
mRNA Refseq: NM_000412
Protein Refseq: NP_000403
UniProt ID: P04196
Chromosome Location: 3q27
MIM: 142640
Pathway: Hemostasis
Function: cysteine-type endopeptidase inhibitor activity; heparin binding; serine-type endopeptidase inhibitor activity

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