Recombinant Human EIF4G1, GST-tagged

• Cat.No.: EIF4G1-28550TH
• Product Overview: Recombinant Human EIF4G1(1500 a.a. - 1599 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 36.74 kDa
• AA Sequence: DVAVLKARAKLLQKYLCDEQKELQA LYALQALVVTLEQPPNLLRMFFDAL YDEDVVKEDAFYSWESSKDPAEQQG KGVALKSVTAFFKWLREAEEESDHN
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: EIF4G1 eukaryotic translation initiation factor 4 gamma, 1 [ Homo sapiens (human) ]
• Official Symbol: EIF4G1
• Synonyms: EIF4G1; eukaryotic translation initiation factor 4 gamma, 1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; eukaryotic translation initiation factor 4 gamma 1; EIF4-gamma; eIF-4-gamma 1; eucaryotic translation initiation factor 4G
• Gene ID: 1981
• mRNA Refseq: NM_182917
• Protein Refseq: NP_886553
• MIM: 600495
• UniProt ID: Q04637
• Chromosome Location: 3q27.1
• Pathway: AUF1 (hnRNP D0) destabilizes mRNA; Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S; Antiviral mechanism by IFN-stimulated genes
• Function: poly(A) RNA binding; protein binding; translation factor activity, nucleic acid binding