Recombinant Human EIF5 protein, GST-tagged

• Cat.No.: EIF5-27177TH
• Product Overview: Recombinant Human EIF5(1 a.a. - 431 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 73.15 kDa
• AA Sequence: MSVNVNRSVSDQFYRYKMPRLIAKV EGKGNGIKTVIVNMVDVAKALNRPP TYPTKYFGCELGAQTQFDVKNDRYI VNGSHEANKLQDMLDGFIKKFVLCP ECENPETDLHVNPKKQTIGNSCKAC GYRGMLDTHHKLCTFILKNPPENSD SGTGKKEKEKKNRKGKDKENGSVSS SETPPPPPPPNEINPPPHTMEEEED DDWGEDTTEEAQRRRMDEISDHAKV LTLSDDLERTIEERVNILFDFVKKK KEEGVIDSSDKEIVAEAERLDVKAM GPLVLTEVLFNEKIREQIKKYRRHF LRFCHNNKKAKRYLLHGLECVVAMH QAQLISKIPHILKEMYDADLLEEEV IISWSEKASKKYVSKELAKEIRVKA EPFIKWLKEAEEESSGGEEEDEDEN IEVVYSKAASVPKVETVKSDNKDDD IDIDAI
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: EIF5 eukaryotic translation initiation factor 5 [ Homo sapiens ]
• Official Symbol: EIF5
• Synonyms: EIF5; eukaryotic translation initiation factor 5; eIF-5; EIF-5A
• Gene ID: 1983
• mRNA Refseq: NM_001969
• Protein Refseq: NP_001960
• MIM: 601710
• UniProt ID: P55010
• Chromosome Location: 14q32.32
• Pathway: Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem
• Function: GTP binding; GTPase activity; binding; nucleotide binding; translation factor activity, nucleic acid binding; translation initiation factor activity