||Recombinant human MTPN, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW =15.0 kDa(138aa), confirmed by MALDI-TOF.
||MTPN (myotrophin), an ankyrin repeat protein, is stimulates protein synthesis and cardiomyocyte growth to initiate cardiac hypertrophy by activating the NF-kappaB signaling cascade. This protein plays a potential role in cerebellar morphogenesis and may function in differentiation of cerebellar neurons, particularly of granule cells. Elevated levels of MTPN are expressed inhumandilated cardiomyopathic and ischemic hearts.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MCDKEFMWAL KNGDLDEVKD YVAKGEDVNR TLEGGRKPLH YAADCGQLEI LEFLLLKGAD INAPDKHHIT PLLSAVYEGH VSCVKLLLSK GADKTVKGPDGLTAFEATDN QAIKALLQ
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.