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Recombinant Human CD27 Molecule, Fc-His

Cat. No.: CD27-1592H
Product Overview: Recombinant human CD27 (amino acid residues 1-192) containing the extracellular domain was fused to the carboxy-terminal 6X his-tagged Fc region of human IgG1. The chimeric protein was expressed inNSO cells.
Description: CD27 is a tumor necrosis factor receptor. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It exists as both a disulfide-linked dimer on the cell surface and as a soluble protein found in serum. It binds to ligand CD70 which is expressed on thymic stromal cells and a small subset of activated T cells and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Molecular Weight: The predicted molecular weight of Recombinant Human CD27 is Mr 46.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 60-70 kDa.
Source: NSO Cells.
State Of Matter: Lyophilized.
Purity: >90% by SDS Page and analyzed by silver stain.
Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Endotoxin: <1.0 EU/µg as determined by the LAL method.
Biological Activity: The biological activity of Human CD27 was determined by its ability to inhibit the proliferation of mouse T cells induced by recombinant mouse CD27 ligand (10 μg/ml, 100 μl/well) and a sub-optimal concentration of anti-CD3. The expected ED50 is typically 0.75 - 3 μg/ml.
Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Gene Name: CD27 CD27 molecule [ Homo sapiens ]
Synonyms: CD27; CD27 antigen; T14; S152; TNFRSF7; MGC20393; Tp55; CD27 molecule; tumor necrosis factor receptor superfamily, member 7; CD27L receptor; T cell activation antigen CD27; T cell antivation antigen S152; T- cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7
Gene ID: 939
mRNA Refseq: NM_001242
Protein Refseq: NP_001233
MIM: 186711
UniProt ID: P26842
Chromosome Location: 12p13
Function: caspase inhibitor activity; protein binding; transmembrane receptor activity

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