Recombinant Human GCLC

• Cat.No.: GCLC-28159TH
• Product Overview: Recombinant full length Human GCLC with a proprietary tag; Predicted MWt 96.14.

Specification

• Description: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
• Protein length: 637 amino acids
• Molecular Weight: 96.140kDa inclusive of tags
• Source: Wheat germ
• Biological activity: useful for Antibody Production and Protein Array
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.79% Tris HCl, 0.31% GlutathioneNote: Glutathione is reduced
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the glutamate--cysteine ligase type 3 family.

Gene Information

• Gene Name: GCLC glutamate-cysteine ligase, catalytic subunit [ Homo sapiens ]
• Official Symbol: GCLC
• Synonyms: GCLC; glutamate-cysteine ligase, catalytic subunit; GLCL, GLCLC; glutamate--cysteine ligase catalytic subunit; GCS
• Gene ID: 2729
• mRNA Refseq: NM_001197115
• Protein Refseq: NP_001184044
• MIM: 606857
• Uniprot ID: P48506
• Chromosome Location: 6p12
• Pathway: Biological oxidations, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, conserved biosystem
• Function: ADP binding; ATP binding; coenzyme binding; glutamate binding; glutamate-cysteine ligase activity