| Species : |
Human |
| Source : |
Mammalian Cells |
| Tag : |
Non |
| Description : |
Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. It also plays a role in embryonic stem cell proliferation. |
| Molecular Weight : |
The predicted molecular weight of Recombinant Human Desmoglein-2 is Mr 89 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 115 kDa. |
| Amino Acid Sequence : |
Human Desmoglein2 (Ala49 Gly608) - IEGRMD - Human IgG1 (Pro100 Lys330). |
| State Of Matter : |
Lyophilized. |
| Purity : |
>95% by SDS Page and analyzed by silver stain. |
| Formulation : |
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2-7.3 with no calcium, magnesium, or preservatives. |
| Endotoxin : |
<1.0 EU/µg as determined by the LAL method. |
| Biological Activity : |
The biological activity of Human DSG2 was determined by the ability of the immobilized protein to support the adhesion of BUD8 human skin fibroblast cells. When 3 x 104 cells are added to recombinant human Desmoglein 2 coated plates (10 μg/ml, 100 μl/well), approximately 50%-80% will adhere after 1 hour at 37°C. |
| Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70℃. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
| Publications : |
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