Recombinant Human NUDT5, His-tagged

• Cat.No.: NUDT5-770H
• Product Overview: Recombinant human NUDT5 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. NW = 26.5 kDa (239aa), confirmed by MALDI-TOF (Real molecular weight on SDS-PAGE will be shift up).

Specification

• Cat. No.: NUDT5-770H
• Description: NUDT5 is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NUDT5 hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium, and also hydrolyzes other nucleotide sugars with low activity such as ADP-glucose and diadenosine diphosphate. As a nudix hydrolase, NUDT5 contains a central nudix motif and functions to eliminate toxic nucleotide metabolites from the cell while maintaining the levels of signaling nucleotides. NUDT5 is widely expressed but is most abundant in liver as a homodimer.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
• Purity: > 85% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NUDT5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 [ Homo sapiens ]
• Synonyms: NUDT5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; YSA1; YSA1H; hYSAH1; ADP-sugar pyrophosphatase; nudix motif 5; nucleoside diphosphate-linked moiety X motif 5; nucleoside diphosphate linked moiety X-type motif 5; EC 3.6.1.13; EC 3.6.1.-
• Gene ID: 11164
• mRNA Refseq: NM_014142
• Protein Refseq: NP_054861
• MIM: 609230
• UniProt ID: Q9UKK9
• Chromosome Location: 10p14
• Pathway: Purine metabolism
• Function: ADP-ribose diphosphatase activity; ADP-sugar diphosphatase activity; hydrolase activity; magnesium ion binding