Active Recombinant Human GSR protein

• Cat.No.: GSR-29083TH
• Product Overview: Recombinant Human GSR Purified from E.coli expressing the human Glutathione Reductase gene (44-522aa with additional 3 amino acids-GSH at N-term)

Specification

• Description: Glutathione reductase (GR) is a member of pyridine nucleotide-disulfide oxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ⇔ 2GSH + NADP+ In most eukaryotic cells, GR maintains the ratio of [GSH]/[GSSG] elevated, and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
• Source: E. coli
• Species: Human
• Form: Lyophilized from 20mM HEPES, pH 7.5
• Bio-activity: 19.4U/mg
• Molecular Mass: 51.7 kDa
• Purity: > 95% by SDS-PAGE
• Unit Definition: One unit will reduce 1μmole of oxidized glutathione per min at pH 7.5 at 25 centigrade
• Storage: Shipped at 4 centigrade. Store at -20 centigrade. After reconstitution, store at -80 centigrade and avoid frequent freeze and thaw.

Gene Information

• Gene Name: GSR glutathione reductase [ Homo sapiens ]
• Official Symbol: GSR
• Synonyms: GSR; glutathione reductase; glutathione reductase, mitochondrial; GR; GRase; MGC78522
• Gene ID: 2936
• mRNA Refseq: NM_000637
• Protein Refseq: NP_000628
• MIM: 138300
• UniProt ID: P00390
• Chromosome Location: 8p21.1
• Pathway: Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; Selenium Pathway, organism-specific biosystem
• Function: NADP binding; electron carrier activity; flavin adenine dinucleotide binding; glutathione binding; glutathione-disulfide reductase activity; oxidoreductase activity; protein homodimerization activity