Recombinant Human GTF2F1, His-tagged

• Cat.No.: GTF2F1-29170TH
• Product Overview: Recombinant full length human GTF2F1 with His tag, isolated from an E. coli strain that carries the coding sequence of the protein under the control of a T7 promoter. amino acids 1-517, approximately 59kDa.

Specification

• Description: The transcription factor IIF (TFIIF) is a heterodimer, composed of 58 kDa (RAP74) and 26 kDa (RAP30) subunits. It was first identified through the ability to interact with immobilized RNA Polymerase II.
• Conjugation: HIS
• Source: E. coli
• Biological activity: 1 unit equals 1 nanogram of purified protein.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the TFIIF alpha subunit family.

Gene Information

• Gene Name: GTF2F1 general transcription factor IIF, polypeptide 1, 74kDa [ Homo sapiens ]
• Official Symbol: GTF2F1
• Synonyms: GTF2F1; general transcription factor IIF, polypeptide 1, 74kDa; general transcription factor IIF, polypeptide 1 (74kD subunit); general transcription factor IIF subunit 1; BTF4; RAP74; TF2F1; TFIIF
• Gene ID: 2962
• mRNA Refseq: NM_002096
• Protein Refseq: NP_002087
• MIM: 189968
• Uniprot ID: P35269
• Chromosome Location: 19p13.3
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem
• Function: DNA binding; catalytic activity; phosphatase activator activity; protein binding; transcription coactivator activity