Recombinant Human HARS, His-tagged

• Cat.No.: HARS-29181TH
• Product Overview: Recombinant full length Human HARS with an N terminal His tag; 532 amino acids with tag, Predicted MWt 59.4 kDa.

Specification

• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
• Protein length: 509 amino acids
• Conjugation: HIS
• Molecular Weight: 59.400kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGSMA ERAALEELVKLQGERVRGLKQQKAS AELIEEEVAKLLKLKAQLGPDESKQ KFVLKTPKGTRDYSPRQMAVREKVF DVIIRCFKRHGAEVIDTPVFELKET LMGKYGEDSKLIYDLKDQGGELLSL RYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFD IAGNFDPMIPDAECLKIMCEILSSL QIGDFLVKVNDRRILDGMFAICGVS DSKFRTICSSVDKLDKVSWEEVKNE MVGEKGLAPEVADRIGDYVQQHGGV SLVEQLLQDPKLSQNKQALEGLGDL KLLFEYLTLFGIDDKISFDLSLARG LDYYTGVIYEAVLLQTPAQAGEEPL GVGSVAAGGRYDGLVGMFDPKGRKV PCVGLSIGVERIFSIVEQRLEALEE KIRTTETQVLVASAQKKLLEERLKL VSELWDAGIKAELLYKKNPKLLNQL QYCEEAGIPLVAIIGEQELKDGVIK LRSVTSREEVDVRREDLVEEIKRRT GQPLCIC

Gene Information

• Gene Name: HARS histidyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: HARS
• Synonyms: HARS; histidyl-tRNA synthetase; histidyl-tRNA synthetase, cytoplasmic; histidine tRNA ligase 1; cytoplasmic
• Gene ID: 3035
• mRNA Refseq: NM_002109
• Protein Refseq: NP_002100
• MIM: 142810
• Uniprot ID: P12081
• Chromosome Location: 5q31.3
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; histidine-tRNA ligase activity; ligase activity; nucleotide binding