Recombinant Human Intercellular Adhesion Molecule 1, Fc Chimera

Cat. No.: ICAM1-1625H
Product Overview: Recombinant Human ICAM-1 was expressed inNSO cells.The extracellular domain of human ICAM-1 (amino acid residues 1 - 480) was fused to the carboxy-terminal Fc region of human IgG1 via a polypeptide linker.
Description: Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.
Source: NSO Cells.
State Of Matter: Lyophilized.
Purity: >95% by SDS Page and analyzed by silver stain.
Endotoxin: <1.0 EU/µg as determined by the LAL method.
Molecular Weight: The predicted molecular weight of Recombinant Human ICAM-1 is Mr 70 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 110-125 kDa.
Formulation: The recombinant protein was 0.2 μm filtered and lyophilized from PBS.
Biological Activity: The biological activity of Human ICAM-1 was determined by the ability of the immobilized protein to support the adhesion of HSB2 cells, a human peripheral blood acute lymphoblastic leukemia cell line. When 5 x 104 cells/well are added to recombinant human ICAM/Fc coated plates (12.5 μg/ml with 100 μl/well), approximately 60 - 70% will adhere after PMA stimulation for 1 hour at RT.
Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Gene Name: ICAM1 intercellular adhesion molecule 1 [ Homo sapiens ]
Synonyms: ICAM1; intercellular adhesion molecule 1; BB2; CD54; P3.58; human rhinovirus receptor; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54 antigen; cell surface glycoprotein P3.58; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
Gene ID: 3383
mRNA Refseq: NM_000201
Protein Refseq: NP_000192
MIM: 147840
UniProt ID: P05362
Chromosome Location: 19p13.3-p13.2
Pathway: Cell adhesion molecules (CAMs); Leukocyte transendothelial migration; Natural killer cell mediated cytotoxicity; Integrin cell surface interactions; Signaling in Immune system
Function: integrin binding ; protein binding ; transmembrane receptor activity

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