Recombinant Human LIG1, His-tagged

• Cat.No.: LIG1-26930TH
• Product Overview: Recombinant fragment, corresponding to amino acids 484-919 of Human DNA Ligase I with a N terminal His tag; predicted MWt 50kDa:

Specification

• Description: LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process.Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitution with 96 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: GKGKTAEARKTWLEEQGMILKQTFC EVPDLDRIIPVLLEH GLERLPEHCKLSPGIPLKPMLAHPT RGISEVLKRFEEA AFTCEYKYDGQRAQIHALEGGEVKI FSRNQEDNTGKYP DIISRIPKIKLPSVTSFILDTEAVA WDREKKQIQPFQVLT TRKRKEVDASEIQVQVCLYAFDLIY LNGESLVREPLSR RRQLLRENFVETEGEFVFATSLDTK DIEQIAEFLEQSV KDSCEGLMVKTLDVDATYEIAKRSH NWLKLKKDYLDGV GDTLDLVVIGAYLGRGKRAGRYGGF LLASYDEDSEELQAI CKLGTGFSDEELEEHHQSLKALVLP SPRPYVRIDGAVI PDHWLDPSAVWEVKCADLSLSPIYP AARGLVDSDKGIS LRFPRFIRVREDKQPEQATTSAQVA CLYRKQSQIQNQQGEDSGSDPEDTY

Gene Information

• Gene Name: LIG1 ligase I, DNA, ATP-dependent [ Homo sapiens ]
• Official Symbol: LIG1
• Synonyms: LIG1; ligase I, DNA, ATP-dependent; DNA ligase 1
• Gene ID: 3978
• mRNA Refseq: NM_000234
• Protein Refseq: NP_000225
• MIM: 126391
• Uniprot ID: P18858
• Chromosome Location: 19q13.2-q13.3
• Pathway: Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem
• Function: ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; ligase activity