||Recombinant fragment, corresponding to amino acids 545-863 of Human MCM4 with N terminal His tag; 319 amino acids, 37kDa.
||The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
||Lyophilised:Reconstitute with 87 μl aqua dest.
||Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
||Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
|Sequences of amino acids:
||AYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTR SVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIES QWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMP RLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLI RLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRT GIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTP ALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLT VTGKTVRLL
||Belongs to the MCM family.Contains 1 MCM domain.