Recombinant Human MCM5, His-tagged

• Cat.No.: MCM5-28041TH
• Product Overview: Recombinant fragment, corresponding to amino acids 217-734 of Human MCM5 with N terminal His tag; 518 amino acids, 63kDa.

Specification

• Description: The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 57 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: DKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDK VVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYI RVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVY EVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRR GDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSS AAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFD KMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAA NSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNE ERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIA YCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDR RSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALR LFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLK RRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRR GEIQHRMQRKVLYRLK
• Sequence Similarities: Belongs to the MCM family.Contains 1 MCM domain.

Gene Information

• Gene Name: MCM5 minichromosome maintenance complex component 5 [ Homo sapiens ]
• Official Symbol: MCM5
• Synonyms: MCM5; minichromosome maintenance complex component 5; CDC46, MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) , minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46); DNA replication licensing
• Gene ID: 4174
• mRNA Refseq: NM_006739
• Protein Refseq: NP_006730
• MIM: 602696
• Uniprot ID: P33992
• Chromosome Location: 22q13.1-q13.2
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem
• Function: ATP binding; DNA binding; helicase activity; hydrolase activity; nucleotide binding