Recombinant Human NEK2

• Cat.No.: NEK2-29279TH
• Product Overview: Recombinant full length NEK2 protein (Human), was expressed by baculovirus in Sf9 insect cells using a N-terminal tag, MW 76kDa.

Specification

• Description: This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
• Tissue specificity: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: NEK2 NIMA (never in mitosis gene a)-related kinase 2 [ Homo sapiens ]
• Official Symbol: NEK2
• Synonyms: NEK2; NIMA (never in mitosis gene a)-related kinase 2; serine/threonine-protein kinase Nek2; HsPK 21; NEK2A; NLK1
• Gene ID: 4751
• mRNA Refseq: NM_001204182
• Protein Refseq: NP_001191111
• MIM: 604043
• Uniprot ID: P51955
• Chromosome Location: 1q32-q42
• Pathway: Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity