Recombinant Human NIT2, His-tagged

• Cat.No.: NIT2-26965TH
• Product Overview: Recombinant full length Human NIT2 (amino acids 1-276) with an N terminal His tag (299 amino acids with the tag); predicted mwt: 33 kDa.

Specification

• Description: Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity.
• Protein length: 276 amino acids
• Conjugation: HIS
• Molecular Weight: 33.000kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGSMT SFRLALIQLQISSIKSDNVTRACSF IREAATQGAKIVSLPECFNSPYGAK YFPEYAEKIPGESTQKLSEVAKECS IYLIGGSIPEEDAGKLYNTCAVFGP DGTLLAKYRKIHLFDIDVPGKITFQ ESKTLSPGDSFSTFDTPYCRVGLGI CYDMRFAELAQIYAQRGCQLLVYPG AFNLTTGPAHWELLQRSRAVDNQVY VATASPARDDKASYVAWGHSTVVNP WGEVLAKAGTEEAIVYSDIDLKKLA EIRQQIPVFRQKRSDLYAVEMKKP
• Sequence Similarities: Belongs to the UPF0012 family.Contains 1 CN hydrolase domain.

Gene Information

• Gene Name: NIT2 nitrilase family, member 2 [ Homo sapiens ]
• Official Symbol: NIT2
• Synonyms: NIT2; nitrilase family, member 2; omega-amidase NIT2
• Gene ID: 56954
• mRNA Refseq: NM_020202
• Protein Refseq: NP_064587
• Uniprot ID: Q9NQR4
• Chromosome Location: 3q12.3
• Pathway: Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem
• Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; molecular_function; omega-amidase activity