Recombinant Human PAK2

• Cat.No.: PAK2-30670TH
• Product Overview: Recombinant fragment, amino acids 227-524 of Human PAK2, expressed in E.coli, 33.35 kDa,

Specification

• Description: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
• Protein length: 298 amino acids
• Molecular Weight: 33.350kDa
• Source: E. coli
• Biological activity: 86 nmoles/min/μgAnalysis of enzymatic activity: 1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR20) at room temperature for 1 hour
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: PAK2 p21 protein (Cdc42/Rac)-activated kinase 2 [ Homo sapiens ]
• Official Symbol: PAK2
• Synonyms: PAK2; p21 protein (Cdc42/Rac)-activated kinase 2; p21 (CDKN1A) activated kinase 2; serine/threonine-protein kinase PAK 2; PAK65; PAKgamma; S6/H4 kinase
• Gene ID: 5062
• mRNA Refseq: NM_002577
• Protein Refseq: NP_002568
• MIM: 605022
• Uniprot ID: Q13177
• Chromosome Location: 3q29
• Pathway: Activation of Rac, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Axon guidance, organism-specific biosystem
• Function: ATP binding; identical protein binding; nucleotide binding; protein binding; protein kinase activity