Recombinant Human TNFRSF14, Fc-His

• Cat.No.: TNFRSF14-1691H
• Product Overview: Recombinant Human TNFRSF14 was expressed in a mouse myeloma cell line,NSO.The DNA sequence encoding the extracellular domain of human HVEM was fused to the carboxy-terminal 6X histidine-tagged region of human IgG1 via a linker peptide and inserted into a suitable expression vector.

Specification

• Cat. No.: TNFRSF14-1691H
• Description: Tumor necrosis factor receptor superfamily member 14 (herpesvirus entry mediator), also known as TNFRSF14, is the cellular receptor for TNF superfamily 14 (LIGHT). TNFRSF14 is a type I membrane protein that is considered as a Herpesvirus entry mediator. It is able to inhibit the proliferation, activation, and cytokine production of T cells. TNFRSF14 is expressed by peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells.
• Molecular Weight: The predicted molecular weight of Recombinant Human TNFRSF14 is Mr 45 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 60 kDa.
• Source: Mammalian cells
• Amino Acid Sequence: N-Terminus: human HVEM (Met 1 - Val 202); IEGRMD Human IgG1 (Pro 100 - Lys 330); HHHHHH C-Terminus.
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Endotoxin: <0.1 ng per 1 µg as determined by the LAL method.
• Biological Activity: The biological activity of Human HVEM-Fc was determined by its ability to inhibit apoptosis of mouse L-929 cells treated with 0.1 ng/mL of rhTNF-β. The expected ED50 for this effect is typically 0.5 - 2 μg/mL.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: TNFRSF14 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) [ Homo sapiens ]
• Synonyms: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator); TR2; ATAR; HVEA; HVEM; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily, member 14; CD40-like protein; herpesvirus entry mediator A; tumor necrosis factor receptor-like gene2; OTTHUMP00000000866; Herpesvirus entry mediator A; Tumor necrosis factor receptor-like 2
• Gene ID: 8764
• mRNA Refseq: NM_003820
• Protein Refseq: NP_003811
• MIM: 602746
• UniProt ID: Q92956
• Chromosome Location: 1p36.3-p36.2
• Pathway: Cytokine-cytokine receptor interaction; Signaling in Immune system
• Function: tumor necrosis factor receptor activity; transmembrane receptor activity; protein binding