Recombinant Human TNFRSF18, Fc Chimera

• Cat.No.: TNFRSF18-1621H
• Product Overview: Recombinant Human TNFRSF18 encoding the signal peptide from hCD33 (Met 1 - Ala 16) joined with the extracellular domain of human GITR (amino acid residues 26 - 161) was fused to the Fc region of human IgG1 via a polypeptide linker and expressed inNSO cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 45307 a.a.
• Description: GITR (glucocorticoid-induced tumor necrosis factor receptor) also known as TNFRSF18, is a surface receptor molecule that has been shown to be involved in inhibiting the suppressive activity of T-regulatory cells and extending the survival of T-effector cells.1 GITR is involved in the regulation of T-cell receptor mediated cell death. Constitutive expression of a transfected GITR gene induces resistance to apoptosis induced by anti CD3 monoclonal antibodies. GITR ligand interferes with the suppressor activity of CD4(+) CD25(+) regulatory T-cells.
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human GITR is Mr 41 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 50 kDa.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Biological Activity: Measured by its ability to bind GITR ligand. Immobilized Human GITR at 2 μg/ml (100 μl/well) can bind Human GITR ligand with a linear range of 0.3 - 20 ng/ml in a functional ELISA assay.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: TNFRSF18 tumor necrosis factor receptor superfamily, member 18 [ Homo sapiens ]
• Synonyms: tumor necrosis factor receptor superfamily, member 18; AITR; GITR; GITR-D; TNFRSF18; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; OTTHUMP00000001519; OTTHUMP00000001520; OTTHUMP00000001521; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein
• Gene ID: 8784
• mRNA Refseq: NM_004195
• Protein Refseq: NP_004186
• MIM: 603905
• UniProt ID: Q9Y5U5
• Chromosome Location: 1p36.3
• Pathway: Cytokine-cytokine receptor interaction
• Function: receptor activity; tumor necrosis factor receptor activity