Recombinant Human PDE6A

• Cat.No.: PDE6A-30821TH
• Product Overview: Recombinant fragment of Human PDE6 alpha with an N terminal proprietary tag, expressed by Baculovirus in Sf9 insect cells system; predicted mwt: 120kDa.

Specification

• Description: This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
• Protein length: 827 amino acids
• Molecular Weight: 120.000kDa inclusive of tags
• Source: Baculovirus
• Biological activity: the specific activity of PDE6 alpha was determined to be 38 nmol/min/mg
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: PDE6A phosphodiesterase 6A, cGMP-specific, rod, alpha [ Homo sapiens ]
• Official Symbol: PDE6A
• Synonyms: PDE6A; phosphodiesterase 6A, cGMP-specific, rod, alpha; PDEA; rod cGMP-specific 3,5-cyclic phosphodiesterase subunit alpha
• Gene ID: 5145
• mRNA Refseq: NM_000440
• Protein Refseq: NP_000431
• MIM: 180071
• Uniprot ID: P16499
• Chromosome Location: 5q31.2-q34
• Pathway: Hemostasis, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Phototransduction, organism-specific biosystem; Phototransduction, conserved biosystem; Platelet homeostasis, organism-specific biosystem
• Function: 3,5-cyclic-GMP phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity