Recombinant Human WNT3A

• Cat.No.: WNT3A-1700H

Specification

• Cat. No.: WNT3A-1700H
• Description: Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule. WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm. Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells. It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state. Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis.
• Source: CHO.
• State Of Matter: Lyophilized.
• Purity: >75% as determined by SDS-PAGE under reducing conditions.
• Endotoxin: <0.1 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human WNT-3a (Ser19 - Lys352) is Mr 37.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40 kDa.
• Formulation: The protein was 0.2 µm filtered and lyophilized from PBS, 1.0 mM EDTA, 0.5% CHAPS, pH 6.8.
• Biological Activity: The biological activity of Human WNT-3a was determined by its ability to promote alkaline phosphatase production by MC3T3E1 cells. The expected ED50 is 5-25 ng/ml.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C for up to three months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Publication:
  Fibrinogen Induces RUNX2 Activity and Osteogenic Development from Human Pluripotent Stem Cells. (2016)
  Lineage-specific differentiation of osteogenic progenitors from pluripotent stem cells reveals the FGF1-RUNX2 association in neural crest-derived osteoprogenitors (2020)

Gene Information

• Gene Name: WNT3A wingless-type MMTV integration site family, member 3A [ Homo sapiens ]
• Synonyms: wingless-type MMTV integration site family, member 3A; MGC119418; MGC119419; MGC119420; WNT3A; OTTHUMP00000035713
• Gene ID: 89780
• mRNA Refseq: NM_033131
• Protein Refseq: NP_149122
• MIM: 606359
• UniProt ID: P56704
• Chromosome Location: 1q42
• Pathway: Basal cell carcinoma; Hedgehog signaling pathway; Melanogenesis; Pathways in cancer; Wnt signaling pathway
• Function: extracellular matrix structural constituent; protein binding; signal transducer activity