Active Recombinant Human ARAF (YY301, 302DD) Mutation Protein, GST/His-tagged

• Cat.No.: ARAF-504H
• Product Overview: Recombinant human serine/threonine kinase ARAF, encompassing amino acids 282-606 (end), contains mutations Y301D and Y302D was expressed in Sf9 insect cells. The construct contains a GST-tag at the N-terminal end as well as a C-terminal His-tag (6xhis). The protein was affinity purified.

Specification

• Description: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Insect cell
• Species: Human
• Tag: GST/His
• Form: Aqueous buffer solution
• Bio-activity: 6 pmol/min/μg.
• Molecular Mass: 63 kDa
• Protein length: aRAF (GST-282-606(end)-His)
• Purity: 61%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at -80 centigrade.
• Storage Buffer: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
• Shipping: At -80 centigrade

Gene Information

• Gene Name: ARAF A-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: ARAF
• Synonyms: ARAF; A-Raf proto-oncogene, serine/threonine kinase; PKS2; A-RAF; ARAF1; RAFA1; serine/threonine-protein kinase A-Raf; A-Raf proto-oncogene serine/threonine-protein kinase; Oncogene ARAF1; Ras-binding protein DA-Raf; proto-oncogene A-Raf-1; proto-oncogene Pks; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; EC 2.7.11.1
• Gene ID: 369
• mRNA Refseq: NM_001654
• Protein Refseq: NP_001645
• MIM: 311010
• UniProt ID: P10398