Recombinant Human ARAF Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ARAF-3790H
• Product Overview: ARAF MS Standard C13 and N15-labeled recombinant protein (NP_001645) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 67.6 kDa
• AA Sequence: MEPPRGPPANGAEPSRAVGTVKVYL PNKQRTVVTVRDGMSVYDSLDKALK VRGLNQDCCVVYRLIKGRKTVTAWD TAIAPLDGEELIVEVLEDVPLTMHN FVRKTFFSLAFCDFCLKFLFHGFRC QTCGYKFHQHCSSKVPTVCVDMSTN RQQFYHSVQDLSGGSRQHEAPSNRP LNELLTPQGPSPRTQHCDPEHFPFP APANAPLQRIRSTSTPNVHMVSTTA PMDSNLIQLTGQSFSTDAAGSRGGS DGTPRGSPSPASVSSGRKSPHSKSP AEQRERKSLADDKKKVKNLGYRDSG YYWEVPPSEVQLLKRIGTGSFGTVF RGRWHGDVAVKVLKVSQPTAEQAQA FKNEMQVLRKTRHVNILLFMGFMTR PGFAIITQWCEGSSLYHHLHVADTR FDMVQLIDVARQTAQGMDYLHAKNI IHRDLKSNNIFLHEGLTVKIGDFGL ATVKTRWSGAQPLEQPSGSVLWMAA EVIRMQDPNPYSFQSDVYAYGVVLY ELMTGSLPYSHIGCRDQIIFMVGRG YLSPDLSKISSNCPKAMRRLLSDCL KFQREERPLFPQILATIELLQRSLP KIERSASEPSLHRTQADELPACLLS AARLVPTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ARAF v-raf murine sarcoma 3611 viral oncogene homolog [ Homo sapiens (human) ]
• Official Symbol: ARAF
• Synonyms: ARAF; v-raf murine sarcoma 3611 viral oncogene homolog; ARAF1, v raf murine sarcoma 3611 viral oncogene homolog 1; serine/threonine-protein kinase A-Raf; Oncogene ARAF1; proto-oncogene Pks; proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; A-Raf proto-oncogene serine/threonine-protein kinase; PKS2; A-RAF; ARAF1; RAFA1
• Gene ID: 369
• mRNA Refseq: NM_001654
• Protein Refseq: NP_001645
• MIM: 311010
• UniProt ID: P10398