Recombinant Human PRKAR1B, His-tagged

• Cat.No.: PRKAR1B-29537TH
• Product Overview: Recombinant full length Human PKA regulatory subunit I beta with a C terminal His tag expressed in Sf9 insect cells; 52 kDa inclusive of tag

Specification

• Description: Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.
• Protein length: 381 amino acids
• Conjugation: HIS
• Molecular Weight: 52.000kDa inclusive of tags
• Tissue specificity: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM Tris HCl, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the cAMP-dependent kinase regulatory chain family.Contains 2 cyclic nucleotide-binding domains.

Gene Information

• Gene Name: PRKAR1B protein kinase, cAMP-dependent, regulatory, type I, beta [ Homo sapiens ]
• Official Symbol: PRKAR1B
• Synonyms: PRKAR1B; protein kinase, cAMP-dependent, regulatory, type I, beta; cAMP-dependent protein kinase type I-beta regulatory subunit
• Gene ID: 5575
• mRNA Refseq: NM_001164758
• Protein Refseq: NP_001158230
• MIM: 176911
• Uniprot ID: P31321
• Chromosome Location: 7pter-p22
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Aquaporin-mediated transport, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem
• Function: cAMP binding; cAMP-dependent protein kinase regulator activity; nucleotide binding