Recombinant Human PRMT1, MBP-tagged

• Cat.No.: PRMT1-31114TH
• Product Overview: Recombinant full length Human PRMT1 fused to a His-MBP tag at the N-terminus; 750 amino acids, MWt 84 kDa.

Specification

• Description: The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).
• Conjugation: MBP
• Source: E. coli
• Biological activity: Specific Activity: 10,000unit/ml.Unit Definition : One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37C.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 40% Glycerol, 40mM Tris, 4mM Magnesium chloride, 2mM DTT, 100mM Sodium chloride, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTGIKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQSGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEALSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQEPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGLTFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAWSNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPNKELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELAKDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAASGRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAAAEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFGIHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGILCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTIIKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYARDKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYGFDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVKVEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGFSTSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNAKNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

Gene Information

• Gene Name: PRMT1 protein arginine methyltransferase 1 [ Homo sapiens ]
• Official Symbol: PRMT1
• Synonyms: PRMT1; protein arginine methyltransferase 1; HMT1 (hnRNP methyltransferase, S. cerevisiae) like 2 , HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) , HRMT1L2; protein arginine N-methyltransferase 1; ANM1; HCP1
• Gene ID: 3276
• mRNA Refseq: NM_001207042
• Protein Refseq: NP_001193971
• MIM: 602950
• Uniprot ID: Q99873
• Chromosome Location: 19q13
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Tryptophan metabolism, organism-specific biosystem; mRNA processing, organism-specific biosystem
• Function: N-methyltransferase activity; N-methyltransferase activity; histone methyltransferase activity; histone methyltransferase activity (H4-R3 specific); methyltransferase activity