||The DNA sequence encoding the signal peptide from CD33 (Met 1 - Ala 16), fused to the carboxy-terminal 6X his-tagged mature mouse Gas6 (Asp 115 - Pro 673) lacking the Gla domain was expressed inNSO cells.
||Growth arrest-specific 6, also known as GAS6 is a growth factor that serves as a ligand for members of the Axl subfamily of receptor protein-tyrosine kinases. GAS6 is expressed in hematopoietic tissue. GAS6 function as a direct regulator of osteoclastic function; they give an insight into the role of these vitamin K-dependent ligands in bone resorption in vivo. GAS6 has a potential therapeutic target to minimize graft-versus-host disease (GVHD).
||The predicted molecular weight of Recombinant Mouse Gas6 is Mr 62 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 72 kDa.
|State Of Matter:
||>95% by SDS Page and analyzed by silver stain.
||<1.0 EU/µg as determined by the LAL method.
||The biological activity of Mouse Gas6 was determined by its ability to bind rmAxl/Fc in a functional ELISA. Immobilized rmAxl/Fc at 2 μg/ml (100 μl/well) can bind rmGas6 with a linear range of 0.08 - 5 ng/ml.
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.