Recombinant Human RAD1, His-tagged

• Cat.No.: RAD1-31287TH
• Product Overview: Recombinant full length Human Rad1 with N terminal His tag; 302 amino acids, MWt 33.9kDa.

Specification

• Description: This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described.
• Protein length: 282 amino acids
• Conjugation: HIS
• Molecular Weight: 33.900kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMPLLT QQIQDEDDQYSLVASLDNVRNLSTI LKAIHFREHATCFATKNGIKVTVEN AKCVQANAFIQAGIFQEFKVQEESV TFRINLTVLLDCLSIFGSSPMPGTL TALRMCYQGYGYPLMLFLEEGGVVT VCKINTQEPEETLDFDFCSTNVINK IILQSEGLREAFSELDMTSEVLQIT MSPDKPYFRLSTFGNAGSSHLDYPK DSDLMEAFHCNQTQVNRYKISLLKP STKALVLSCKVSIRTDNRGFLSLQY MIRNEDGQICFVEYYCCPDEEVPES ES
• Sequence Similarities: Belongs to the rad1 family.

Gene Information

• Gene Name: RAD1 RAD1 homolog (S. pombe) [ Homo sapiens ]
• Official Symbol: RAD1
• Synonyms: RAD1; RAD1 homolog (S. pombe); RAD1 (S. pombe) homolog; cell cycle checkpoint protein RAD1; cell cycle checkpoint protein Hrad1; checkpoint control protein HRAD1; DNA repair exonuclease REC1; exonuclease homolog RAD1; HRAD1; Rad1 like DNA damage checkpoin
• Gene ID: 5810
• mRNA Refseq: NM_002853
• Protein Refseq: NP_002844
• MIM: 603153
• Uniprot ID: O60671
• Chromosome Location: 5p13
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; G2/M Checkpoints, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem
• Function: 3-5 exonuclease activity; damaged DNA binding; exodeoxyribonuclease III activity; hydrolase activity; protein binding