Recombinant Human RHEB, T7 -tagged

• Cat.No.: RHEB-31325TH
• Product Overview: Recombinant full length mature human RHEB protein , fused to T7 tag at N terminus; 197 amino acids, 21.7 kDa.

Specification

• Description: This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
• Conjugation: T7
• Tissue specificity: Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYILV YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC
• Sequence Similarities: Belongs to the small GTPase superfamily. Rheb family.

Gene Information

• Gene Name: RHEB Ras homolog enriched in brain [ Homo sapiens ]
• Official Symbol: RHEB
• Synonyms: RHEB; Ras homolog enriched in brain; Ras homolog enriched in brain 2 , RHEB2; GTP-binding protein Rheb
• Gene ID: 6009
• mRNA Refseq: NM_005614
• Protein Refseq: NP_005605
• MIM: 601293
• Uniprot ID: Q15382
• Chromosome Location: 7q36
• Pathway: Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem
• Function: GTP binding; GTPase activity; metal ion binding; nucleotide binding