||Recombinant Mouse Il1rl1 encoding the extracellular domain (amino acid residues Met 1 - Ser 342) was fused to the Fc region of human IgG1 via a polypeptide linker, inserted into a suitable baculovirus expression vector and expressed inSf21 cells.
||ST2 belongs to the immunoglobulin superfamily and bears significant amino acid identity (~25%) to the extracellular portion of both type I and type II interleukin 1 receptors (IL-1R). Some of the intracellular residues that are required for signal transduction through the IL-1R and are conserved in the Drosophila Toll protein are also found in the membrane form of ST2. Furthermore, the gene for ST2 was mapped to mouse chromosome 1 closely linked to the il-1r locus containing both type I and type II receptor genes in support of their common ancestry.
||The predicted molecular weight of Recombinant Mouse IL-1 R4 is Mr 63 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 73 kDa.
||Sf 21 Insect Cells.
|State Of Matter:
||>95% by SDS Page and analyzed by silver stain.
||<0.1 EU/µg as determined by the LAL method.
||The biological activity of Mouse IL-1 R4 was determined by its ability to bind rmIL-33 in a functional ELISA type binding assay. The estimated KD is < 50 nM.
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.