Recombinant Human USO1, His-tagged

• Cat.No.: USO1-31129TH
• Product Overview: Recombinant fragment, corresponding to amino acids 746-962 of Human Vesicle docking protein p115 with an N terminal His tag. Predicted mwt: 25 kDa

Specification

• Description: The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 130 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: QELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSE QVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTE AFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELK NEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITD SKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDE LESGDQEDEDDESEDPGKDLDHI
• Sequence Similarities: Belongs to the VDP/USO1/EDE1 family.Contains 10 ARM repeats.

Gene Information

• Gene Name: USO1 USO1 vesicle docking protein homolog (yeast) [ Homo sapiens ]
• Official Symbol: USO1
• Synonyms: USO1; USO1 vesicle docking protein homolog (yeast); USO1 homolog, vesicle docking protein (yeast); general vesicular transport factor p115; p115; TAP; transcytosis associated protein; VDP; vesicle docking protein
• Gene ID: 8615
• mRNA Refseq: NM_003715
• Protein Refseq: NP_003706
• MIM: 603344
• Uniprot ID: O60763
• Chromosome Location: 4q21.1
• Pathway: Arf1 pathway, organism-specific biosystem
• Function: binding; protein transporter activity