||Recombinant human MAT II beta was expressed inE.coliand purified by using conventional chromatography techniques. MW = 36.4 kDa (323aa).
||Methionine adenosyltransferase II, beta (MAT II beta) belongs to the methionine adenosyltransferase (MAT) family. MAT II beta catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Its expression in hepatoma cell lines may lead to increase DNA synthesis and thereby participate to cell proliferation.
|Amino Acid Sequence:
||MPEMPEDMEQ EEVNIPNRRV LVTGATGLLG RAVHKEFQQN NWHAVGCGFR RARPKFEQVN LLDSNAVHHI IHDFQPHVIV HCAAERRPDV VENQPDAASQ LNVDASGNLA KEAAAVGAFL IYISSDYVFD GTNPPYREED IPAPLNLYGK TKLDGEKAVL ENNLGAAVLR IPILYGEVEK LEESAVTVMF DKVQFSNKSA NMDHWQQRFP THVKDVATVC RQLAEKRMLD PSIKGTFHWS GNEQMTKYEM ACAIADAFNL PSSHLRPITD SPVLGAQRPR NTQLDCSKLE TLGIGQRTPF RIGIKESLWP FLIDKRWRQT VFH
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 1mM EDTA, 10% glycerol.
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.